[DIYbio] Re: On protein expression in eukaryotes

What is always helpful: adding the N-terminus of a native protein to the N-terminus of your intended protein...

Expression signals, ribosome ramp, codon usage, stability... All enhanced if you choose the right protein... 

Like add the first (7-15) amino acids of (Rubisco is a bad example because it has to be stable in the chloroplast rather than in the cytosolic environment) ... a very stable protein in the cytosol to your protein...

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