Do you know of any papers on this N-terminus phenomenon? I have never heard of it.
On Thu, Mar 13, 2014 at 4:40 AM, Mega [Andreas Stuermer] <masterstorm123@gmail.com> wrote:
What is always helpful: adding the N-terminus of a native protein to the N-terminus of your intended protein...Expression signals, ribosome ramp, codon usage, stability... All enhanced if you choose the right protein...Like add the first (7-15) amino acids of (Rubisco is a bad example because it has to be stable in the chloroplast rather than in the cytosolic environment) ... a very stable protein in the cytosol to your protein...--To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/791fb26e-df4b-4938-a143-d4964dc8c252%40googlegroups.com.
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