Re: [DIYbio] Storing DNA

On Wed, Mar 19, 2014 at 3:36 PM, Cathal Garvey
<cathalgarvey@cathalgarvey.me> wrote:
> I've seen differing opinions there, that when using TE you should keep
> in fridge rather than freezer. The alleged reasons were:
> 1) It's extremely stable at 4C with TE to scavenge divalent cations anyway.

Hmm, DNA storage at 4C? I have not heard of that before. How long do
you think this is possible for?

> 2) Freezing risks creating hypersaline (eg low freezing point) pockets
> as ice crystal formation excludes TE salts, which can then fluctuate
> between frozen and unfrozen states fairly easily..this can act like a
> shredder in the medium term on DNA and actually decrease stability.
>

I sometimes have to use low concentrations of DNA for qpcr
(femtograms). To reduce loss to tube walls, I prepare dilutions in TE
with 100x BSA. As to long term storage, I have found that the DNA
seems to degrade and fresh dilutions are preferable. Your explanation
sounds reasonable.

On Thu, Mar 20, 2014 at 8:42 AM, SC <stacy734@yahoo.com> wrote:
> I keep plasmids in 0.1 TE, which is recommended by the sequencing lab. I've
> had no problems with degradation and the sequences come out OK.
>

I will have to keep that in mind. I use 1x TE. I wonder how much of a
difference there is for long term stability. By the way, what
sequencing lab do you use?

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