RE: [DIYbio] Storing DNA

I know invitrogen sells "genestrings" which save costs by letting u do
the subcloning. They are lyophilized fragments. Maybe that's what he
meant by artificial genes in a vial. TE buffer worked well for me. Have
some plasmids and ready to go PCR fragments at -20 for four years and
no detectable issues. I even stored some in holy water (ultra pure
depec treated) in same conditions and nothing out of the ordinary. More
important I think is dnase activity than temp. Its a very stable
molecule after all.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D From: Jeswin
Sent: 3/19/2014 1:30 PM
To: diybio@googlegroups.com
Subject: Re: [DIYbio] Storing DNA
On Wed, Mar 12, 2014 at 10:06 PM, Jordan Fryer <jfrex2010@gmail.com> wrote:
> What sort of equipment would you use to store artificial genes in vials? I
> thought a -20 C freezer would be good for this, but I need your opinions.
>

What exactly do you mean by "artificial genes in vials"?

Plasmids, PCR products, gDNA in buffer (TE, etc) at -20C
Lyophilized oligos can be kept at 4C

I think I have some plasmids at -80C and they've been there for many years now.

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