[DIYbio] kitchen DNA extraction not showing on the gels

Extraction of DNA from tomatoes for running in 2% agarose/EtBr gel

86 g tomato

100 mL deionized water

5 mL detergent

25g salt

12g Sodium citrate

ice cold isopropanol

5 mL TE buffer

loading dye

ladder

extraction

1) chop and pulp tomato and put in sandwich bag

2) add 100 mL deionized water, 5 mL detergent, 25g salt to bag

3) squash and mix tomato in bag and leave for 20 mins

4) strain liquid through gauze into test tube, fill to about ¼

5) gently fill the test tube to ¾ full with ice cold isopropanol and leave for 15 minutes

6) tried a few variations here; (none of which worked)

i) isolate the pellet of red precipitate that floated to the top

redissolve in TE buffer and add 1/5 loading dye to make a 1/6 dilution

ii) spin down a sample from the white cloudy stuff that has formed at the top of the alcohol layer

redissolve in TE buffer and add 1/5 loading dye to make a 1/6 dilution

7) run the extracted DNA with loading dye;

8) we are seeing the ladder lanes showing up in the UV, and the loading dye is running, but the sample lanes are completely empty.

There is a very faint suggestion of a glow in the well, but hardly anything

What are we doing wrong?