On 21 April 2014 00:56, Dakota Hamill <dkotes@gmail.com> wrote:
This was the state of the current gel. It has 2 ladders and the rest are various extracted potions. You can see that the wells have some UV response.
Maybe I missed it but, what DNA stain are you using? gel green?
Ethidium Bromide
You're extracting genomic DNA with probably a lot of contamination, and who knows how much of it. If anything, you should only see a glow in the wells, and depending on how much of it there is and how chopped up it is, maybe a smear below the wells. If I repeated the experiment I'd consider it a success if I saw a glow in the wells as that's where the massive pieces of DNA you extract should be, probably still in tight cahoots with some histones or maybe other proteins that don't let the DNA dye get close enough to bind efficiently for a good UV response.
This was the state of the current gel. It has 2 ladders and the rest are various extracted potions. You can see that the wells have some UV response.
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