Re: [DIYbio] My DIY Gene Gun worked!!

WHOA Yes! Congrats Sebastian, can't wait to build one!

On 19 April 2014 19:00:13 GMT+01:00, Sebastian Cocioba <scocioba@gmail.com> wrote:
Here is a picture of a plant leaf expressing gfp shot using tungsten nanoparticles using my home made gene gun. Ill write a decent post on my blog about it but I was too excited to wait. This was 48hrs post shot. Tobacco leaf, 35s-gfp-Tnos cassette. Left leaf is experiment, right leaf is control. Not the glow in the midrib. Leaf illuminated with party light black light.

Cost of gene gun was $30-50 depending on shipping (plumbing parts and bike pump), tungsten is $38/5g from US Nano, not counting the calcium or spermidine on looking at $0.01/shot (0.5mg) and all done at room temp!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D


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Sent from my Android device with K-9 Mail. Please excuse my brevity.

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