We do at LAB I'll be there in 20 minutes.
Just take some mastermix, leave it at room temperature for a week, then take it out and test it against mastermix that has been frozen all that time.--Anyone got any mastermix?
On Saturday, April 26, 2014 11:06:58 PM UTC-7, aaronvollrath wrote:>>The thing is that most enzymes especially polymerases can last at RT. They are thermostable.... and most restriction enzymes are used at 37C and they don't die. I have never actually tested how most restriction enzymes will react but i definitely will and I think it will be on the positive side. That they don't need to be shipped cold.
"A larger trial of 23 different restriction enzymes held at room temperature for one week showed that all enzymes retained significant activity. As a practical demonstration of the usefulness of this finding, enzymes were posted to Africa by conventional mail (cost $1 US) and shown to retain activity upon arrival after three weeks in transit (compared to a cost of $1000 US by cold-chain transportation)"
http://www.biotechniques.com/multimedia/archive/00011/ 00293st06_11772a.pdf On Fri, Apr 25, 2014 at 4:11 PM, Josiah Zayner <josiah...@gmail.com> wrote:The thing is that most enzymes especially polymerases can last at RT. They are thermostable.... and most restriction enzymes are used at 37C and they don't die. I have never actually tested how most restriction enzymes will react but i definitely will and I think it will be on the positive side. That they don't need to be shipped cold.I have a Taq strain and plasmid that I will put it on the website but purchasing enzymes is just really cheap and I can't compete with the prices that suppliers have.Also, no time for testing at the moment. But I would be glad to host plasmids and strains just give me a month or two to work it out.
On Fri, Apr 25, 2014 at 3:49 PM, Koeng <koen...@gmail.com> wrote:
Btw when it comes to enzymes I actually have a few expression strains for common enzymes that use the maltose binding protein if you are interested. I never tested them though, got distracted with other projects.I have SpeI (not cloned yet), T5 exonuclease, Taq, and I think i have T4 ligase somewhere around. (Was cloning all the enzymes for gibson assembly/ biobricks)Also i am thinking of synthesizing pfu polymerase, so if you purify enzymes in house I would be willing to send ya an expression strain. (with a trade of some of the produced enzymes)-Koeng
On Friday, April 25, 2014 2:42:55 PM UTC-7, Josiah Zayner wrote:The plasmid.strain stock is great idea! I have been talking to people about making this happen because the $65 of addgene is awful.
Currently, I am just offering ones I think would be useful. I also have pUC19 and many others and obviously the list will grow.
I think allowing people to sell their own plasmids.strains would be great and hopefully I can make that happen later in the year.
I am attempting to keep the prices low at the moment to make things available so I don't want $50 plasmids... yet. At least until things get established and I have a workflow.
Because this is a registered.licensed company if you need specific chemicals or something that is non-hazardous I would be glad to purchase it and ship it to you. I think that is the biggest problem with Science. Companies not even willing to ship NaCl to a residential address because of some guidelines made by a board of directors that have never done Science, that make no sense to me.
On Friday, April 25, 2014 2:15:14 PM UTC-7, Nathan McCorkle wrote:On Fri, Apr 25, 2014 at 2:11 PM, Koeng <koen...@gmail.com> wrote:
> if you need a 10bp ladder, can't you just order a 10bp primer and run that?
a 10bp ladder would mean fragments of increasing length in increments
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