The thing is that most enzymes especially polymerases can last at RT. They are thermostable.... and most restriction enzymes are used at 37C and they don't die. I have never actually tested how most restriction enzymes will react but i definitely will and I think it will be on the positive side. That they don't need to be shipped cold.
I have a Taq strain and plasmid that I will put it on the website but purchasing enzymes is just really cheap and I can't compete with the prices that suppliers have.
Also, no time for testing at the moment. But I would be glad to host plasmids and strains just give me a month or two to work it out.
On Fri, Apr 25, 2014 at 3:49 PM, Koeng <koeng101@gmail.com> wrote:
Btw when it comes to enzymes I actually have a few expression strains for common enzymes that use the maltose binding protein if you are interested. I never tested them though, got distracted with other projects.I have SpeI (not cloned yet), T5 exonuclease, Taq, and I think i have T4 ligase somewhere around. (Was cloning all the enzymes for gibson assembly/ biobricks)Also i am thinking of synthesizing pfu polymerase, so if you purify enzymes in house I would be willing to send ya an expression strain. (with a trade of some of the produced enzymes)-Koeng
On Friday, April 25, 2014 2:42:55 PM UTC-7, Josiah Zayner wrote:The plasmid.strain stock is great idea! I have been talking to people about making this happen because the $65 of addgene is awful.
Currently, I am just offering ones I think would be useful. I also have pUC19 and many others and obviously the list will grow.
I think allowing people to sell their own plasmids.strains would be great and hopefully I can make that happen later in the year.
I am attempting to keep the prices low at the moment to make things available so I don't want $50 plasmids... yet. At least until things get established and I have a workflow.
Because this is a registered.licensed company if you need specific chemicals or something that is non-hazardous I would be glad to purchase it and ship it to you. I think that is the biggest problem with Science. Companies not even willing to ship NaCl to a residential address because of some guidelines made by a board of directors that have never done Science, that make no sense to me.
On Friday, April 25, 2014 2:15:14 PM UTC-7, Nathan McCorkle wrote:On Fri, Apr 25, 2014 at 2:11 PM, Koeng <koen...@gmail.com> wrote:
> if you need a 10bp ladder, can't you just order a 10bp primer and run that?
a 10bp ladder would mean fragments of increasing length in increments
of 10bp. so 10 to probably 100 or 150bp.To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/40f952a7-1e68-4f0a-aa2f-5cb1670687f9%40googlegroups.com.--
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