Re: [DIYbio] Re: Transgenic CCD resistant honeybee - need help

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Agricultural GM Regulation in US doesn't only apply to edibles, it
applies to anything relevant to agriculture. Bees definitely qualify,
even ignoring the fact that they make honey, because they are critical
pollinators.

So yes, you'll hit a huge regulatory barrier that you'll have to
overcome with years of high quality testing.

This is a huge project. If you can pull off the actual science, which is
already very ambitious (but not impossible), that's about a third of the
work. The second third is getting approval to actually bring it to
market, and the third third is getting people to trust it enough to
actually use and accept it.

That's all assuming that it's even possible for the product of your
scientific labours to get regulatory approval, because if your
implementation of CCD-resistant bees isn't contain-able they'll probably
refuse permission. Same for if it's so over-contained that it poses a
risk to other bee populations, see "Sterile Male". Sure, sterile drones
makes for a useful (but incomplete) way to contain your GM bees. It also
risks interfering with the mating behaviour of other mating swarms of
unmodified bees; have you studied virgin queen behaviour? How many
drones will a bee of each breed in use in the US typically mate with on
her virgin flight? If ~1-4, your sterile drones might lead to failed
matings if they DDOS the queen.

So, don't ignore the need for LOTS OF MONEY, because anything big
requires money when facing nigh-insurmountable obstacles like the
regulatory burden of proving your bees won't pose more of a nuisance
than a solution. If people are offering you low-strings grant money to
fix problems, take the money and do the thing.

On 05/05/14 20:01, Mega [Andreas Stuermer] wrote:
>> This is a very cool project idea, but I think you are going to encounter a
> lot of roadblocks. Even here in the US, which is kind of the wild west of
> GMOs, the FDA and EPA do assessments of >potential environmental and human
> health impact for anything that will be released and/or consumed.
>
> With the glowing plant there was no regulation, unless it was an edible
> plant.
> And what I heard, US are more liberal with transgenic animals than plants.
>
>
>> This is expensive and lengthy, for animals more so than plants. The GMO
> salmon still haven't made it to market after 10+ years. It's going to be
> even more difficult to get regulatory approval when >you're adding a ton of
> new genes.
>
> A ton = 4 :D at first it will be only GFP and three resistance genes
> against viruses and fungi.
> If I can show a GFP bee, some investors will probably jump on the train. I
> hope so at least, I am willing to take the risk.
>
>
>> Also, do you have any precedent for adding so many resistance genes to an
> insect?
> As for bees, no. 2 genes at the same time.
> For other beetles, Idk. Nobody tries to make insects resistant. You usually
> want to kill them.
>
> >Are they contained in a single piece of DNA, and if so will it be able to
> get into the sperm?
> Yes. And perhaps. But the probosed mechanism is that the DNA is attached to
> the sperms surface and gets inside the cell, as soon as they fuse.
>
> On the other hand my construct is very small. I made it to put the 4 genes
> into 2100 bp. The original sperm-mediated paper also had at least 1500 bp
> (I didn't count it but GFP ~800 promoter ~500) and they also took
> terminator etc.
>
>
>> How high does the expression need to be for these genes to effective?
> This has the potential to put stress on the cell metabolism, and make the
> bees less healthy.
> Not very much, probably. I chose a medium promoter.
>
>> I won't say walk before you run, I think you're already running. But
> maybe run more before you do an ultra-triathlon.
> I love mega projects :D
>

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