[DIYbio] Re: firefly luciferase regenerating enzyme

It's just luciferin *regenerating* enzyme, not producing. Thus you still have to add luciferin.
 
AFAIK, they have done in the US both firefly luciferase (probably plus LRE) for a photo and the main work is how to implement vibrio fischeri lux.
 
 
 
 
 

On Wednesday, June 4, 2014 7:35:49 PM UTC+2, poli wrote:
Anyone have any info on expression of the firefly LRE in plants in combination with luciferase? The cambridge igem team performed their work in e coli and found d-cysteine was a necessary component. The glowing plant project has conflicting info on which luciferase system they are using and I haven't seen any publications except Krichevsky et al 2010 Plos One (who used vibrio). Anyone know if coexpression of LRE with luciferase has been shown to regenerate any luciferin without addition of d-cysteine?

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