[DIYbio] Re: firefly luciferase regenerating enzyme

I have luciferase expressed in my algae which I feed luciferin (this works well), I would like to work with the regenerating enzyme if there is some indication it works under normal cell conditions to *regenerate* the spent luciferin (and to further test my system). Luciferase timecourses show a diminished signal over a time which I would like to combat. The igem team seemed to find d-cysteine is necessary to regenerate the correct chiral luciferin and I want to know from if this is necessary in vivo in a eukaryote. Some claim d-cysteine is present in the peroxisome and the luciferase + LRE tend to localize there, or luciferase can change the chirality of l-luciferase to d-luciferase. I could express the LRE with luciferase in the algae with d-cysteine and chbt in the media and screen for luciferase signal to see if it works but I don't want to spend $300 and a month of work before feeling out what does and doesn't work for others. Anyone express LRE and show it works, if so under what conditions?