Re: [DIYbio] Cheaply freezing Wisdom tooth

>11 bucks a year for -80 freezing

Plus shipment cost to america 1000$ overnight on ice? :D

(just guesstimating)

 

Plus, will they be functional after such long delivery on ice? Somwhere I read, though, that they were still cultivable after 5 days.

 

 

>Why exactly do you want to freeze the tooth anyway? 

http://www.ncbi.nlm.nih.gov/pubmed/21417716

These Japanese researchers are conducting a human trial at the moment. They take pulp from a tooth, put it into a nectrotic tooth and after 14 days it is vital again (regrowth of blood vessels and nerves)

 

I would really hate throwing those valuable stem cells away.

 

 

On Thu, Jun 5, 2014 at 5:32 PM, Koeng <koeng101@gmail.com> wrote:

https://www.transcriptic.com/pricing/

11 bucks a year for -80 freezing

On Thursday, June 5, 2014 8:01:15 AM UTC-7, Mega [Andreas Stuermer] wrote:

I have got no -80°C freezer and I don't think university will allow me to throw it into theirs :P

On Thu, Jun 5, 2014 at 3:15 PM, Dakota Hamill <dko...@gmail.com> wrote:

Snap freeze in liquid nitrogen then Negative 80 maybe?  Or just plan your experiment and when you're ready bust out the pliers and rip out a new root sample.  That'd be pretty hardcore though.

I could picture a diy'er pointing to missing teeth saying this experiment failed, worked, worked, failed.   Maybe there is a business opportunity in frozen teeth, get on it!

On Jun 5, 2014 8:59 AM, "Mega [Andreas Stuermer]" <masters...@gmail.com> wrote:
>
> Hi everyone.
>
> Anyone got an idea how to freeze Wisdom teeth cheaply? I asked at a in-vitro-fertilization bank and at the Red Cross. The first said "absolutely no" while the second one said "rather no" perhaps quick and dirty, and 100€ per year.
>  
> Isn't there a cheaper way? What if I use -20°C? The cells probably won't be functional after few months right?
>  
> Any input appreciated ;)
>
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