Re: [DIYbio] Cheaply freezing Wisdom tooth

I have got no -80°C freezer and I don't think university will allow me to throw it into theirs :P

On Thu, Jun 5, 2014 at 3:15 PM, Dakota Hamill <dko...@gmail.com> wrote:

Snap freeze in liquid nitrogen then Negative 80 maybe?  Or just plan your experiment and when you're ready bust out the pliers and rip out a new root sample.  That'd be pretty hardcore though.

I could picture a diy'er pointing to missing teeth saying this experiment failed, worked, worked, failed.   Maybe there is a business opportunity in frozen teeth, get on it!

On Jun 5, 2014 8:59 AM, "Mega [Andreas Stuermer]" <masters...@gmail.com> wrote:
>
> Hi everyone.
>
> Anyone got an idea how to freeze Wisdom teeth cheaply? I asked at a in-vitro-fertilization bank and at the Red Cross. The first said "absolutely no" while the second one said "rather no" perhaps quick and dirty, and 100€ per year.
>  
> Isn't there a cheaper way? What if I use -20°C? The cells probably won't be functional after few months right?
>  
> Any input appreciated ;)
>
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http://diyspartanbiotech.files.wordpress.com/2014/03/genetic-engineering-and-synbio-for-beginners-v1.pdf