Re: [DIYbio] Low cost PCR machine

Hi Subin! Namaste!

Where are you from in India? At least which state?

There are a few hackerspaces and some people that might not have a
hackerspace local, but that post on this list once in a while... I
believe at least one person in Bangalore, and a hackerspace that is
working to get electronics equipment and a regular meeting/hacking
space (an office or somewhere safe to store equipment) in Dharamsala
(http://hillhacks.in/)

Glad to hear you're interested and doing some projects! There is a lot
of learning ahead if you want to do really cool and/or cutting edge
stuff, but it IS possible!

On Tue, Jun 3, 2014 at 12:13 PM, Subin <subinedu@gmail.com> wrote:
> Hi guys,
> Myself subin a engineering student in biotechnlogy. I am
> actually new to this community("call me a new bourn"),just 19 year old.For
> about a month i was working on a project called "Low cost PCR".A normal PCR
> machine costs about 2- 3 lack in india. So i wished i should make one of
> myown. The principles are simple so it is simple to convert into a code and
> make it work. I used an arduino uno do the coding,LM 35 temparture ic as a
> sensor.I do works on Robotics so it was not a difficult to combine biology
> and electronics.
>
> Ill give the arduino code i have written for My PCR hope it might be useful
> for you. I will update its solid works model and circuit stuffs in my blog.
> My design will aprox cost about 16 dollers
> This is the arduino code.....i have used a header file called timer.h
>
> #include "Timer.h"
> Timer t;
> const int fan = 12;
> const int bulb = 11;
> const int temp_sensor =1;
> int sensor_value;
> int temp;
> int number;
> int count = 1;
> void setup()
> {
> pinMode(fan,OUTPUT);
> pinMode(bulb,OUTPUT);
> Serial.begin(9600);
> number = Serial.read();
> while(count <= number)
> {
> void heating_up();
> int denaturation_clock = t.every(60000, denaturation);
> Serial.println(denaturation_clock);
> int annealing_clock = t.every(45000, annealing);
> Serial.println(annealing_clock);
> int extention_clock = t.every(120000, extention);
> Serial.println(extention_clock);
> count++;
> }
> }
> void loop()
> {
> }
> int temparature()
> {
> sensor_value=analogRead(temp_sensor);
> temp=sensor_value*0.48828125;
> return(temp);
> }
> void heating_up()
> {
> temparature();
> while(temp != 94)
> {
> heating();
> }
> }
> void heating()
> {
> digitalWrite(fan,LOW);
> digitalWrite(bulb,HIGH);
> }
> void cooling()
> {
> digitalWrite(bulb,LOW);
> digitalWrite(fan,HIGH);
> }
> void denaturation()
> {
> temparature();
> while(temp == 93)
> {
> digitalWrite(bulb,LOW);
> digitalWrite(fan,HIGH);
> }
> heating();
> }
> void annealing()
> {
> temparature();
> while(temp <= 52);
> {
> digitalWrite(bulb,HIGH);
> digitalWrite(fan,LOW);
> }
> cooling();
> }
> void extention()
> {
> temparature();
> while(temp >= 72)
> {
> cooling();
> }
> heating();
> }
>
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--
-Nathan

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