Re: [DIYbio] Low cost PCR machine

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There are *lots* of interesting people in Bangalore interested in
biohacking, he'd be lucky to live there! :)

On 05/06/14 02:52, Nathan McCorkle wrote:
> Hi Subin! Namaste!
>
> Where are you from in India? At least which state?
>
> There are a few hackerspaces and some people that might not have a
> hackerspace local, but that post on this list once in a while... I
> believe at least one person in Bangalore, and a hackerspace that is
> working to get electronics equipment and a regular meeting/hacking
> space (an office or somewhere safe to store equipment) in Dharamsala
> (http://hillhacks.in/)
>
> Glad to hear you're interested and doing some projects! There is a lot
> of learning ahead if you want to do really cool and/or cutting edge
> stuff, but it IS possible!
>
> On Tue, Jun 3, 2014 at 12:13 PM, Subin <subinedu@gmail.com> wrote:
>> Hi guys,
>> Myself subin a engineering student in biotechnlogy. I am
>> actually new to this community("call me a new bourn"),just 19 year old.For
>> about a month i was working on a project called "Low cost PCR".A normal PCR
>> machine costs about 2- 3 lack in india. So i wished i should make one of
>> myown. The principles are simple so it is simple to convert into a code and
>> make it work. I used an arduino uno do the coding,LM 35 temparture ic as a
>> sensor.I do works on Robotics so it was not a difficult to combine biology
>> and electronics.
>>
>> Ill give the arduino code i have written for My PCR hope it might be useful
>> for you. I will update its solid works model and circuit stuffs in my blog.
>> My design will aprox cost about 16 dollers
>> This is the arduino code.....i have used a header file called timer.h
>>
>> #include "Timer.h"
>> Timer t;
>> const int fan = 12;
>> const int bulb = 11;
>> const int temp_sensor =1;
>> int sensor_value;
>> int temp;
>> int number;
>> int count = 1;
>> void setup()
>> {
>> pinMode(fan,OUTPUT);
>> pinMode(bulb,OUTPUT);
>> Serial.begin(9600);
>> number = Serial.read();
>> while(count <= number)
>> {
>> void heating_up();
>> int denaturation_clock = t.every(60000, denaturation);
>> Serial.println(denaturation_clock);
>> int annealing_clock = t.every(45000, annealing);
>> Serial.println(annealing_clock);
>> int extention_clock = t.every(120000, extention);
>> Serial.println(extention_clock);
>> count++;
>> }
>> }
>> void loop()
>> {
>> }
>> int temparature()
>> {
>> sensor_value=analogRead(temp_sensor);
>> temp=sensor_value*0.48828125;
>> return(temp);
>> }
>> void heating_up()
>> {
>> temparature();
>> while(temp != 94)
>> {
>> heating();
>> }
>> }
>> void heating()
>> {
>> digitalWrite(fan,LOW);
>> digitalWrite(bulb,HIGH);
>> }
>> void cooling()
>> {
>> digitalWrite(bulb,LOW);
>> digitalWrite(fan,HIGH);
>> }
>> void denaturation()
>> {
>> temparature();
>> while(temp == 93)
>> {
>> digitalWrite(bulb,LOW);
>> digitalWrite(fan,HIGH);
>> }
>> heating();
>> }
>> void annealing()
>> {
>> temparature();
>> while(temp <= 52);
>> {
>> digitalWrite(bulb,HIGH);
>> digitalWrite(fan,LOW);
>> }
>> cooling();
>> }
>> void extention()
>> {
>> temparature();
>> while(temp >= 72)
>> {
>> cooling();
>> }
>> heating();
>> }
>>
>> --
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>> To view this discussion on the web visit
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>
>
>

--
T: @onetruecathal, @IndieBBDNA
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