Re: [DIYbio] thermal cyclers for PCR

On 06/04/2014 01:20 PM, Jebus Jones wrote:
> It depends how big the air gap is. There would of course be an optimum.

Just remove the air gap.

Removing the air gap by having air be the cooling fluid is the optimum
for low costs and high performance. Thin walled vials would be compatible with
air cooling/heating. Any kind of thick vials, or vials dropped in holes
in blocks of metal, (as was done in PCR machines of 1995), is going to cost more
and probably be lower performance.

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