Hi. I'm in the process of buying equipment to set up a basic "DIY bio" lab. I have most of the items needed, but I haven't been able to afford a proper centrifuge yet. The "Dremelfuge" and similar DIY setups are certainly interesting, but I am not comfortable relying on such solutions because of safety concerns. So I'm wondering - is there a cheaper alternative to centrifugation for pelleting/harvesting bacterial cultures? Does anyone have experience with using filtering for cell culture harvesting? I did a quick search, and found this one article as an example: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC291564/
-- Is filtration a reasonable alternative to centrifugation for cell culture harvesting in DIY bio labs?
A second question: I do have a benchtop "ultramicro"-centrifuge for spinning down Eppendorf tubes. Does anyone have experience with using these with spin columns? Can the lower RPM be offset by longer centrifugation times, or are they just too weak?
Best regards,
Jarle Pahr
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