[DIYbio] Re: DNA Synthesizer under development

Hey,

Sorry for the late answer, we ve been very busy in the last days.

The chemistry is mainly based on phosphoramidite synthesis. Our approach is to guide MCPG in a magnetic field to the reagents, instead of a static solid support. Liquid delivery us carried out by syringe pumps.
We are expecting similar write lenghts as classic column based synthesizers. In a second step we are planning to fully automate OE-PCR and link it to the synthesizer or integrate it into the chip.


PDMS does slightly swell, that's why we are also working with SIFEL which doesn't.  Silicone tubing might be replaced by PTFE or Fluran F-5500-A tubing.


Am Dienstag, 24. Juni 2014 06:57:23 UTC+1 schrieb Gene Hacker:

Could you provide a basic outline of how you intend to build a DNA synthesizer? What exactly are you using magnetic beads for? And do you really think you'll be able to achieve kilobase write lengths?

We use uv-cureable polymers and printed masks for the mold, PDMS & SIFEL (fluorinated silicon) for the chip, vakuum pumps to degas the PDMS  and corona treatment to bond the PDMS to glass.

Doesn't PDMS experience swelling when used with most solvents?