Quick question:
Would a terminator sequence work if cloned in reverse?
I have primers for amplifying the nopaline synthase terminator but in my new vector the two cutters in question are in the reverse order. If I clone it in, it would be "antisense" to the proper sequence. Since terminators, in general, are just hairpin physical structures, would the reverse still work in eukaryotes?
Im making a combinatorial set of my transient expression vector for use in testing my gene gun using various promoters and terminators and so it would be great if I can save some money on primers and explore the effects of a reverse terminator on overall expression rate. Just wanted to see if anyone has some experience before I waste the few bucks on cloning the terminator in. Thanks!
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
Would a terminator sequence work if cloned in reverse?
I have primers for amplifying the nopaline synthase terminator but in my new vector the two cutters in question are in the reverse order. If I clone it in, it would be "antisense" to the proper sequence. Since terminators, in general, are just hairpin physical structures, would the reverse still work in eukaryotes?
Im making a combinatorial set of my transient expression vector for use in testing my gene gun using various promoters and terminators and so it would be great if I can save some money on primers and explore the effects of a reverse terminator on overall expression rate. Just wanted to see if anyone has some experience before I waste the few bucks on cloning the terminator in. Thanks!
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
0 comments:
Post a Comment