Glad to hear im not the only one crazy enough to buy a 9600! She is built like a tank. Im sure it will outlive me.
How does she fare with tricky or longer amplifications?
Ever run Q5 (98C denat) with it?
I use it for large plant genotyping assays and optimized primers so the machine won't need much precision but never did a cloning run with it for fear of temp drift and all that jazz. Used some of the eBay taq mastermix which I also highly recommend. You can't beat $99 for 2000 10uL reactions! Just search PCR mastermix on eBay. Its sold by Gene and Cell technologies which looks a lot like openbiotech and uses the pOpenTaq vector for production.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
How does she fare with tricky or longer amplifications?
Ever run Q5 (98C denat) with it?
I use it for large plant genotyping assays and optimized primers so the machine won't need much precision but never did a cloning run with it for fear of temp drift and all that jazz. Used some of the eBay taq mastermix which I also highly recommend. You can't beat $99 for 2000 10uL reactions! Just search PCR mastermix on eBay. Its sold by Gene and Cell technologies which looks a lot like openbiotech and uses the pOpenTaq vector for production.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Koeng
Sent: 7/25/2014 12:44 PM
To: diybio@googlegroups.com
Subject: Re: [DIYbio] Available PCR machines - comparisons and experiences?
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