Re: [DIYbio] pJE202 pVIB in liquid culture

If glowing is quorum sensing dependent .... there will be a higher local concentration of inducer with a colony than in liquid culture where the induced can more easily diffuse away.
>matt

Sent from my Verizon Wireless 4G LTE DROID


Josiah Zayner <josiah.zayner@gmail.com> wrote:

To be more specific, when I grow it on a plate I see it fine. But when I grow it in liquid culture alongside the plate I don't see anything. Since the liquid culture is more dense I would expect to see it better? I know in the original paper they added a shit ton of stuff to the culture. Wondering if any of that is actually required? If there is a specific strain of E. coli that works better or worse? If quorum sensing can help or inhibit it(should help?)?

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/7de7c5dd-baa3-4ecf-a0fc-8c1333b72b05%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment