Re: [DIYbio] practical laminar flow benches

On Fri, Jul 18, 2014 at 11:00 AM, John Griessen <john@industromatic.com> wrote:
> What is the goal of laminar lab bench? "Nothing gets out?" " Nothing gets
> in?"
>
> Nothing gets in is the goal -- keeping your cultures sterile and clean
> while allowing some particles or mist of them to flow out.


Actually the goal of laminar flow is that nothing gets IN OR OUT. If
you're designing for nothing to get IN, it is much easier, which is
all Dakota cared about (he specifically mentioned this, but used the
word 'laminar' instead of just 'clean air', likely as that is possibly
where he sourced the unit from).

Laminar flow means a sheet of flowing air, the sheet carries anything
floating inside OR outside into a chamber and past a filter. It is
used for keeping your dirtiness out of your cultures, and keeping your
culture's dirtiness (i.e. infectious particles, to you or other
experiments) inside.

This level of bio has previously been discussed as not really
appropriate for home/DIY work. If you want to pursue this type of
work, you best learn a ton about PPE and BSL levels, infectious waste
handling, etc.

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