Was actually looking online a while ago for such a unit for my own DIY hood. Currently I've built two crude laminar flow boxes (sterile air fan box filter thing...) using a simple rectangular box made of plywood, a 3ft x 1ft x 6in hepa filter (used eBay) and a duct blower fan tied to a light dimmer to get close to 90 cubic feet per minute.
I didn't really bother to test particles or what not since ill be using it exclusively for plant tissue culture, not silicon wafer handling in a clean room. My test was simple: several rounds of Petri dishes filled with various nutrient agar media which would grow a very broad range of bugs, fungi, etc and left them open in the draft overnight then incubated at 37C and room temp.
No growth across any of the five assay (30 dishes per assay) so I deemed it clean enough for my needs.
The laminar flow cabinet (PCR Workstation from AirClean) I use at my day job has a basic setup where the air is blown using what appears to be one of those overhead ceiling hepa fan units similar to what you found, a UV light, and a 2x2x4ft polycarbonate box that has a flap in the front for arms and object manipulation. Unit costs $2500 new but im sure you could make on for cheap. Just throwing in my experience. Hope you manage to build a hood for your enjoyment soon!
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
I didn't really bother to test particles or what not since ill be using it exclusively for plant tissue culture, not silicon wafer handling in a clean room. My test was simple: several rounds of Petri dishes filled with various nutrient agar media which would grow a very broad range of bugs, fungi, etc and left them open in the draft overnight then incubated at 37C and room temp.
No growth across any of the five assay (30 dishes per assay) so I deemed it clean enough for my needs.
The laminar flow cabinet (PCR Workstation from AirClean) I use at my day job has a basic setup where the air is blown using what appears to be one of those overhead ceiling hepa fan units similar to what you found, a UV light, and a 2x2x4ft polycarbonate box that has a flap in the front for arms and object manipulation. Unit costs $2500 new but im sure you could make on for cheap. Just throwing in my experience. Hope you manage to build a hood for your enjoyment soon!
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Dakota Hamill
Sent: 7/18/2014 8:16 PM
To: diybio@googlegroups.com
Subject: Re: [DIYbio] Re: Let's play with high voltage - Laminar flow hood squirrel cage fan
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