You know what? You are right. I didn't realize that you mentioned your expression vector in a context of making a kit for gene delivery or a patent on plasmid.
-Why are you so upset and snarky?
My one liners were snarky?
-- -Why are you so upset and snarky?
My one liners were snarky?
Accept my apology and understand that it was difficult to judge a character when he is cool - composed and then flips a switch when he notices you are on the scene asking questions. Understand where the confusion got me. All I did after that was recognize a pattern with you mentioning your expression vector.
I had no further interest in your vector after you mentioned how painstaking it was to construct the first time you explained it. Your work is your brainchild. I get that. Don't think that I am bitter about it. I wouldn't even share details of my work if I didn't have to rely on advice from others from time to time.
-"publicly salty" what is that? I am afraid I am not familiar with that phrase. Attempts at tracking down the meaning gave me nothing other than cryptography.
-Do you plan on self-experimentation?
Question built on assumptions but I did work you up to those assumptions so that deserves an answer. Not that I would reserve to self-experimentation but I believe that an inventor should have that freedom. My personal interests are with plants. Most you can do with that for self-experimentation is using its extracts or cook it for dinner. Self-experimentation with a DIY biolistics gun or any such delivery system would be a stupid way to go. There are always other modes of gene delivery and they are getting better.
I had no further interest in your vector after you mentioned how painstaking it was to construct the first time you explained it. Your work is your brainchild. I get that. Don't think that I am bitter about it. I wouldn't even share details of my work if I didn't have to rely on advice from others from time to time.
-"publicly salty" what is that? I am afraid I am not familiar with that phrase. Attempts at tracking down the meaning gave me nothing other than cryptography.
-Do you plan on self-experimentation?
Question built on assumptions but I did work you up to those assumptions so that deserves an answer. Not that I would reserve to self-experimentation but I believe that an inventor should have that freedom. My personal interests are with plants. Most you can do with that for self-experimentation is using its extracts or cook it for dinner. Self-experimentation with a DIY biolistics gun or any such delivery system would be a stupid way to go. There are always other modes of gene delivery and they are getting better.
Thanks for the lengthy reply. I had to ask myself, what deserved this kind of reply?
A thought just occurred as I was wrapping this up.
I am paraphrasing but -bacterial and cp expression is similar. I think I understand why you are targeting cp.
A thought just occurred as I was wrapping this up.
I am paraphrasing but -bacterial and cp expression is similar. I think I understand why you are targeting cp.
On Friday, July 18, 2014 6:23:27 AM UTC-4, Sebastian wrote:
That is a gross misinterpretation of my post, yuriy. How you could
possibly link my off-list denial of giving you a vector I've made from
scratch for free or otherwise which contains novel, patentable,
elements not found in prior art which I kindly explained to my on-list
post of my rant about someone wanting to do self-experimentation may
hurt themselves because they think its a good idea to use said gene gun
inappropriately is beyond me. How are the two related? Why are you so
upset and snarky? Do you plan on self-experimentation?
Its quite difficult to convince someone that a rant wasn't intended in
any way towards them but come on now. What did I say that made you
think it had anything to do with you? Im sorry you are upset that I
didn't just hand you months of hard work and heavy financial burden
regardless of compensations offered. I don't think its fair to hold
that against me and twist my words. :( I mean no quarrel, friend and am
happy to help with any and all questions verbosely regarding your
project as I've done previously but I can't just hand out freebies if I
could support myself with them otherwise. Its a moral dilemma and right
now im too broke to throw such plasmid into the public domain yet.
Please refrain from posting personal issues on the list. Its not fair
to anyone. I would have preferred you just contact me in person and
discuss the issues at hand and not be publicly salty about them. I
don't know how else to convince you that you were, in no way, the
target nor even the after thought of my colorfully worded rant about
bio-punk grinders. Hope we can still maintain a positive, professional
relationship and set this issue aside. Peace, love, science.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D From: Yuriy Fazylov
Sent: 7/18/2014 1:42 AM
To: diy...@googlegroups.com
Subject: [DIYbio] Re: On the topic of safety and DIY tools
Good to know it's not directed at anyone. This coming after I asked
you for an expression vector from you and you just happening to
mention it. No need to hide what you mean.
Still I am curious about what could do 1500 psi and cost less than
$100. I walked through a hardware store just out of curiosity :)
I wasn't wrong to ask you for the vector either. Did I noticed a plan
for gene repository here? Nice.
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