RE: [DIYbio] Re: Using bubblewrap to create chemical and bi assays...

Next shipment that comes with decently sized bubble wrap I get ill
squirt some luke warm MS-Agar, let it solidify, and dispense a few
Arabidopsis seeds atop. Wonder what smallest needle gauge to still pass
seeds?

Nail polish should work to seal it, right? Would be interesting to try.
I use eppi tubes to start small seeds for quick DNA processing etc and
reuse the tubes by autoclave. Quick disposable titer plates for testing
mannose isomerase activity would be nice. Just an overnight
colorimeteric assay of small bit of callus tissue.

As for saran vacuum forming, how many sheets do you guys think would be
enough for a decent replica of a standard titer plate?

Ill post pictures if I happen to stumble upon some fun...err...bubble
wrap :P

Very fun ideas, everyone!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D From: Nathan McCorkle
Sent: ‎7/‎18/‎2014 5:27 PM
To: diybio
Subject: Re: [DIYbio] Re: Using bubblewrap to create chemical and bi
assays...
/me envisions some over-excited lab tech jumping on and popping bubble
wrap, until another tech walks in 'my experiments!'

On Fri, Jul 18, 2014 at 2:10 PM, Jonathan Cline <jcline@ieee.org> wrote:
> Three Dee Print a reusable well frame (mold) and vacuum/heat form 0.2 mm
> LDPE film or saran wrap into the wells. 3d printing of plates directly has
> previously been discussed here
> https://groups.google.com/forum/#!topic/diybio/4gzh_PP_hd8 Yes,
> microtiter plates are reusable, depending..
>
>
> You burn your discarded bubble wrap?
>
>
> The upside of using bubble wrap, obviously, is that the bored biologists can
> pop the extras.
>
>
> ## Jonathan Cline
> ## jcline@ieee.org
> ## Mobile: +1-805-617-0223
> ########################
>
> On 7/18/14 12:18 PM, André Esteves wrote:
>
> Have you ever printed something? It's not fast and using supplies for throw
> away stuff it's not rational.
> Imagine that you recycle your plastic... Are recycling contaminated stuff?
>
> Bubble wrap can just burn and with it take away the baddies...
>
> Cheers,
>
> André Esteves
>
>
> 2014-07-18 19:34 GMT+01:00 Jonathan Cline <jncline@gmail.com>:
>>
>> If a 3d printer is already available, why not just print 384-well
>> microtiter plates..
>>
>> ## Jonathan Cline
>> ## jcline@ieee.org
>> ## Mobile: +1-805-617-0223
>> ########################
>>
>>
>> On Friday, July 18, 2014 6:08:19 AM UTC-7, aife wrote:
>>>
>>> Make a frame to hold the bubble wrap and put it in a 3d printer with the
>>> printing head replaced by a programmable syringe and you can do all of
>>> combinatorial interesting things...
>>>
>>
>>
>
>
>
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--
-Nathan

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