Re: [DIYbio] Re: Yuriy's fundraiser on experiment.com

>Usually people use the term "IR" to refer to InfraRed radiation. You might want to clear that up.

I am aware of its usual usage and I did clear that up but papers referencing ionizing radiation often times abbreviate it as such, IR. What drove them to usage of IR (Ionizing Radiation) instead of the commonly known IR (InfraRed) is beyond my understanding. One can only suppose how that happened. Perhaps someone once submitted a paper mentioning "Ionizing Radiation" repetitively and the editor of the journal wrote back condense it. Or maybe someone decided to be a tReNd SeTtEr ;)

Plus there is a limit on how many characters can be used in a section (usually 2K/section) on that website.

>I don't think I have ever heard it referred to as Ionizing Radiation.

I didn't either, until I started looking reading studies/literature on ionizing radiation.

>Also, what type of ionizing radiation due you plan to use? UV? How due you plan to generate the low wavelength UV?

I tracked down a UV-B sensitive response enhancer/promoter system, takes 24 hours to plateau the expression of its protein – 8 hours for its first signs. That means I'll have to grow it, expose it to UV-B, and wait 8-24 hours for it to be tested.

I have yet to look into choline synthase promoters.

I would like to engineer a promoter with promoterCAD with faster response time. I just need to get back to the article on how it runs and see if light response elements in the promoter can just be thrown together.

Plus if I throw in an untested response element into synthesis construct, well let's just say it's not very scientific. I wonder if Project Cyborg is trained to test for something so obscure.

>If alpha, betta, gamma, how do you plan to generate these wavelengths?

From my understanding the fungi can resist up to Gamma but plant allomelanin is not as well characterized as those of the fungus.

Since you are with NASA, you tell me. Where should I solicit help for radiation exposure if the project is gets off the ground? What lab?

>So what is your goal? Why do we need plants that are resistant to ionizing radiation? I think you should clear these things up. Being as specific as possible really helps people understand what you are doing.

Realizing the amount of real estate in space Freeman Dyson once suggested a plant that will live in space and house people. Can you imagine growing space stations? Plants have a lot of properties that can be adjusted but I have never seen dramatic engineered morphogenesis. The closest was removing knots to have finer wood grain. This goal is very conservative compared to space dwelling trees, but I did have a couple of years to think what such biology would require to accommodate itself. My idea was a little more grounded. After writing some things down into what I hoped would be a book I had some computer crashes. That's when I decided not to try to inspire people, that what the concept is for, but to try to do it. I am sure there will be something to be gained from it. Environmental cleanup seems like a valid alternative.

>Also, putting a lot of genes in plants is no easy task. Do you have experience with this?

I wouldn't dream of putting full 20kb synthesis product into any single plant. Although I think the length of a typical Ti vector within biotech use wasn't narrowed down. I wouldn't know what to account for each gene if I did put it all in one vector. Is that what my plan seems like? I will work on the details tonight. Genes and construction on it will have restriction areas and primers in mind. Only the synthesis has to be done at 20kb.

I read and keep reading the literature on these topics. Floral dipping is not as difficult as I first thought but the success rate is off-putting (1%-0.1%). That is unless someone found a better way to do it. It's cheap and fairly easy. You can grow a whole lot on an antibiotic media, or spray seedlings while it's in soil.

After that its all Mendelian genetics.

On Tuesday, July 22, 2014 12:42:10 PM UTC-4, Josiah Zayner wrote:

Hey Yuriy,

Usually people use the term "IR" to refer to InfraRed radiation. I don't think I have ever heard it referred to as Ionizing Radiation. You might want to clear that up. Also, what type of ionizing radiation due you plan to use? UV? How due you plan to generate the low wavelength UV? If alpha, betta, gamma, how do you plan to generate these wavelengths? So what is your goal? Why do we need plants that are resistant to ionizing radiation?

I think you should clear these things up. Being as specific as possible really helps people understand what you are doing.

Also, putting alot of genes in plants is no easy task. Do you have experience with this? If so, mention that.

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