UV or gamma sterilization may also work.
Or you could fill the syringe with bleach, fill the bubble, suck it back out, rinse with sterile medium, and inoculate. It adds time to the procedure, but its automated.
On Fri, Jul 18, 2014 at 10:35 AM, Sebastian Cocioba <scocioba@gmail.com> wrote:
Can someone try it with some SOC media and inccushake it at 37c? Im guessing the air or plastic is hot when made so whatever is trapped had a good chance of dying. Spores may resist. Clean room bubble wrap sounds freaky-expensive.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Nathan McCorkle
Sent: 7/18/2014 12:31 PM
To: diybio
Subject: Re: [DIYbio] Using bubblewrap to create chemical and bi assays...
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/-8246688039678390492%40unknownmsgid.It would suck if you got some bubble wrap contaminated with spores.
I use a heat sealer to package stuff in a clean room, and the plastic roll we use was certified manufactured in a cleanroom too. I wonder if that grade of bubble wrap is produced, and what it costs.
On Jul 18, 2014 6:08 AM, "André Esteves" <aifesteves@gmail.com> wrote:----Make a frame to hold the bubble wrap and put it in a 3d printer with the printing head replaced by a programmable syringe and you can do all of combinatorial interesting things...Apply nail polish with a brush over the holes and you are done...www.newscientist.com/article/dn25916-bubble-wrap-used-for-cheap-blood-and-bacteria-tests.html?utm_source=NSNS&utm_medium=SOC&utm_campaign=facebookgoogletwitter&cmpid=SOC%7CNSNS%7C2012-GLOBAL-facebookgoogletwitter#.U8ka-h_HnFw
Cheers,André Esteves
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