Hello DiyBioists,
-- This is my first (hopefully of many!) contact with the DIYbio community! I plan to drag myself and my lab to a backspace soon, instead of lazing around on the weekends as per!
So, I am currently trying to grow around 10 weird'n'wonderful bacterial species, with the aim of imaging the rotary motor at the base of their flagella using Electron Microscopy. However, I have run into a jam when it comes to reagents. Two of my species' media require a 'vitamin solution' in a very small quantity, and it turns out that this vitamin solution is effectively a concoction of common B vitamins...
To buy these vitamins from sigma and other pro-chem vendors, each is roughy £30-100. This is a hefty sum when I need around 10 vitamins in total to produce the vitamin solution! FYI, this high price is due to the assurance of >99% purity.
However, I have found all the vitamins I require in my local drug store, and the grand total for all the reagents is £30! So my question is, has anyone used vitamins (or any tablet form reagents bought from a drug store) to grow bacteria? Any advice? From what I can tell, the bulking and stabilising agents are all harmless to bacteria... but if anyone knows anything to avoid that would be helpful!
List of bulking agents:
Microcrystaline Cellulose
Dicalcium Phosphate
stearic Acid
Silicon Dioxide
Magnesium Stearate
Cheers!
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