Re: [DIYbio] Buying bacterial reagents from local drug store

Are you going to try to image live and active cells? In an
environmental SEM/TEM? Could you provide some article references if
so?

I don't remember how the videos I've seen of flagella moving were
recorded, I assumed it was fluorescense or some other optical
technique (quick google shows fluoro and dark-field, but not eSEM).

On Sun, Aug 10, 2014 at 11:54 AM, izaak coleman <izaakmalcavoy@gmail.com> wrote:
> Hello DiyBioists,
>
> This is my first (hopefully of many!) contact with the DIYbio community! I
> plan to drag myself and my lab to a backspace soon, instead of lazing around
> on the weekends as per!
>
> So, I am currently trying to grow around 10 weird'n'wonderful bacterial
> species, with the aim of imaging the rotary motor at the base of their
> flagella using Electron Microscopy. However, I have run into a jam when it
> comes to reagents. Two of my species' media require a 'vitamin solution' in
> a very small quantity, and it turns out that this vitamin solution is
> effectively a concoction of common B vitamins...
> To buy these vitamins from sigma and other pro-chem vendors, each is roughy
> £30-100. This is a hefty sum when I need around 10 vitamins in total to
> produce the vitamin solution! FYI, this high price is due to the assurance
> of >99% purity.
>
> However, I have found all the vitamins I require in my local drug store, and
> the grand total for all the reagents is £30! So my question is, has anyone
> used vitamins (or any tablet form reagents bought from a drug store) to grow
> bacteria? Any advice? From what I can tell, the bulking and stabilising
> agents are all harmless to bacteria... but if anyone knows anything to avoid
> that would be helpful!
>
> List of bulking agents:
> Microcrystaline Cellulose
> Dicalcium Phosphate
> stearic Acid
> Silicon Dioxide
> Magnesium Stearate
>
> Cheers!
>
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--
-Nathan

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