This is really more of a science question than a biosafety question anyway.
-- Viruses mutate very rapidly. So assuming you are targeting a bee pathogen, it if highly unlikely the same sequence will be a close match for a human pathogen as well.
Easy enough to check - just blast your sequence against NR, and check the host range for any hits with sequence identity above 70% or so. I highly doubt that you'll trip any alarms unless you're pretty close to a human pathogen.
Patrik
On Thursday, August 21, 2014 2:22:49 PM UTC-7, Nathan McCorkle wrote:
On Thursday, August 21, 2014 2:22:49 PM UTC-7, Nathan McCorkle wrote:
On Thu, Aug 21, 2014 at 2:13 PM, Mega [Andreas Stuermer]
<masters...@gmail.com> wrote:
> Hi!
>
> I was wondering. When I get my construct synthesized that makes bees
> resistant to viruses. Either I use crispr or siRNA - all the methods require
> a reverse complementary sequence to an essential viral protein.
>
> Now, gene synthesis orders are screened not to contain (human?) pathogens,
> and it is good that they do screen to prevent abuse.
So it seems you're implying the viral protein for the bees will be of
a human pathogen?
FWIW the synthesis-screening stuff is all govt recommendations, there
are no requirements. Likewise, there are no requirements for reporting
these incidents. The govt 'recommends' some protocols for screening to
be used, and 'recommends' sequences that are flagged to be cancelled
from synthesis.
Anyway, we're probably all on the list anyway. Or most of us.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/d6295c95-ab09-4b54-b081-1ef694058336%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment