Nope I've had great luck compared to CaCl (40x better) with just water rinsed overnight cell culture:
https://groups.google.com/forum/m/#!topic/diybio/JcKyNIQGLIM
On Aug 6, 2014 8:31 AM, "Mega [Andreas Stuermer]" <masterstorm123@gmail.com> wrote:
-- --You still need a buffer with electrocompetent cells?I mean breeding E coli in LB and adding voltage and DNA won't do the job? You'll need defined conductivity, won'tt you?
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/c2c7e1f9-1293-4767-9742-223254a54b46%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9KqMk2SqRHhjH0XPEZbsciwAGiqR8dA8NnaBxzhHECm2w%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment