Re: [DIYbio] Re: Transgenic CCD resistant honeybee - need help

Nice find on the electroporator! It isn't very high-voltage, and looks
quite easy to build! You could easily swap in an Arduino!

On Mon, Aug 18, 2014 at 11:04 AM, Mega [Andreas Stuermer]
<masterstorm123@gmail.com> wrote:
> Hm, the cheapest gene synthesis provider failed in making the construct.
>
> The next cheapest wants to charge 740$. Is there anyone who wants to be part
> of this and help out with some bucks?
>
>
>
>
>
>
>
> On Monday, April 28, 2014 5:20:42 PM UTC+2, Mega [Andreas Stuermer] wrote:
>>
>> Hi everyone!
>>
>> That latest project I'm currently working on is to confer resistance to a
>> bee.
>>
>> Some of you probably heard of the bee dying due to many different factors,
>> which all together cause CCD.
>> (See http://beeotex.wordpress.com )
>> The bee does kind of inbreeding and therefore has a very small gene pool.
>> Other insects are much better fighting off all the diseases.
>>
>> I found a lot of resistance genes in the literature, such as antibacterial
>> peptides, antifungal peptides, enzymes that destroy insectizides.
>> Additionally, shRNA will fight the worst viruses
>>
>> http://www.ncbi.nlm.nih.gov/pubmed/11122471
>> Here it is shown that you can "easily" transform bees by mixing bee semen
>> with DNA, and fertilizing a queen bee. Some of the offspring will have the
>> DNA then.
>>
>> In this study, the transgenic DNA was inheritable, however it did not
>> integrate into the bees' genomes.
>> Another study showed that a transposon works perfectly to integrate DNA
>> into the honeybee genome (although they did microinjection into larvae and
>> yielded genetic mosaic bees).
>>
>>
>> I have the entire construct and whatsoever, and found a beekeeper in the
>> US who can do the insemination.
>> But DNA 2.0 charges 1800$ for everything (synthesis plus subcloning into
>> this transposon vector).
>>
>> Is there anyone with too much money who would like to help out? In case we
>> will be able to somehow sell the bees, we can offer shares :D
>>
>> Best,
>> Andreas
>>
>>
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--
-Nathan

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