[DIYbio] Re: Karyotyping? etc

Hei,

thanks for your replies!

Well the reason for karyotyping is that the closest I really have to a diagnosis is "gonadal dysgenesis" which could mean anything... eg pais (seems like a probable scenario), etc , so this test would be the way to go so I can narrow down possibilities. I want to know the truth and I want to know why I was sterile and what health problems I could end up facing later.... I think it would be great if there was an easier cheaper way to test for stuff like this, that is if you find out you will have problems with fertility etc, you can plan accordingly.

So in answer to sequencing parts of my dna, allow me to digress and tell a little story, recently one of the masters students came into my lab and we were talking about the bioprinters I work on, he talked about his thesis and how a nanosensor he was developing hadn't worked so I was talking to him about how to get it to work. He looked at me like I was speaking ancient greek until I realized he wasn't a physicist,  he was a chemist!  So going back to the question asked, Josiah, I'm not a biologist, I wouldn't know where to start! Most of my knowledge is in sensors and robotics. I'm merely a dabbler in chemistry and biology but I would like to know more.

Matt : I have access to flo microscopes, I used a confocal microscope for my thesis, however I have to pay to use them here... or rather its taken from the budget.. or I could always bribe someone with chocolate... everyone loves chocolate. :-)

Dakota : thanks for the answer,  my experience with medical professionals is that they always assume they know more than you do and condescendingly dismiss your data. I don't trust medical professionals in general, especially after lying to me and covering up surgeries. The doctors motto I believe is something like " above all else, do no harm" which is a total joke. That's why I'd like to ideally do my own tests and share with others what I have done.

So can anyone point to any papers that illustrate the process? As I'm based in a uni, I can download and share.

Thanx

Jem

On Monday, September 22, 2014 10:22:47 AM UTC+1, jem wrote:

H!

So this is a slightly unusual post. But I was wondering if anyone could offer any advice?

To get to the point, I appear to have been born with an intersex condition, I've had a number of hidden surgeries, etc and I'm still unraveling a bunch of lies having been told to me by the medical profession (and this happens a lot to other's too).

Anywho, I'm looking to do karotyping on myself and perhaps some others... and I was wondering what is the procedure for doing this? I've had a lot of frustrations and difficulties going through the local health service to get these tests done, mainly because they simply don't want to do it as it costs money and is probably deemed by most doctors or endros to be a waste of time and indeed that can be the case, (they also generally don't have a clue about dsds/intersex conditions).  I actually work in a biomedical facility but I'm a physicist primarily, I dabble in tissue engineering growing cells on scaffolds, etc. So I've been asking around here and in the medical school about getting these tests done but I've been told its not so easy, contamination, etc etc 

So does anyone know of any papers / procedures for doing these tests? I have access to every type of equipment here. :-)

Ta

Jem