Re: [DIYbio] Re: Cheapest and easiest way to do PCR at home?

They used this: http://br.mouser.com/ProductDetail/Vishay-Dale/RH050150R0FC02/?qs=oFi4/QsgZXYdykWTBf8Cwn8R2/shRA70

Em terça-feira, 16 de setembro de 2014 14h52min23s UTC-3, biomiky escreveu:

What high power resistors nedded? Like some from an iron?

Il 16/set/2014 19:44 "Otto Heringer" <ottowh...@gmail.com> ha scritto:

Hi,

I'm from Brazil and we're planning to build a open DIYbio lab on our local hackerspace - and as you may see, I'm a newbie here too.

We're planning to build this guy here: http://www.instructables.com/id/Arduino-PCR-thermal-cycler-for-under-85/

OK, you will need a pro acocunt to download the file; and some parts seem to be hard to find. But it could be a nice and cheap project to try out.

Em terça-feira, 9 de setembro de 2014 13h01min01s UTC-3, biomiky escreveu:

I' working on a pcr light bulb project i only need to experiment and assemble. Anybody did it? What do you think about it?

Il 09/set/2014 16:56 "Peter Thielen" <pthi...@gmail.com> ha scritto:

Interesting to note that Genefoo's website went down after your last post, and many of their products are now available on the minipcr website.  These are the same design, but I don't believe Genefoo was actually shipping completed units -- I anticipate that they have joined forces.  

Also of note, any mention of minipcr being open source has now disappeared from their website.  Pricing of individual components for assembling a personalPCR unit was in the $100-125 range last I checked, so the $800 figure has to involve paying people for development, testing, etc.  I don't fault them for the price hike, but I also don't see a lot of individuals making a purchase for a less functional thermocycler than can often be found in the used market for less money.  Support is key, though, so if they manage to make a business out of this, I'll be on the lookout for prices to drop.  

On Saturday, September 6, 2014 11:56:08 PM UTC-4, Sebastian wrote:

I don't mean to offend or step on toes but I am curious: what is the difference between Genefoo's personal pcr and the minipcr. Looks identical but one is a hundred dollars cheaper. I'm really looking into a small pcr machine but that cost can't be justified beyond niche market parameters. Cost of parts plus any reasonable profit margin can't equate to $800 unless the cost is being cranked up due to low demand, or MSRP based on other machines. I mean seriously, a peltier, custom micro, a PID program with thermocouple feedback should be the big cost driver. A custom machined the block may be pricey but not $800. Even OpenPCR shouldn't be that expensive. I really can't see a good way to justify the price. No offense.

On Sep 6, 2014 11:09 PM, "Sebastian Kraves" <bast...@gmail.com> wrote:

check out www.minipcr.com.  Comes assembled, calibrated, and quality tested.

On Tuesday, January 22, 2013 10:44:26 PM UTC-5, Avery Ashley wrote:

What's your setup?

How much did it cost?

Any recommendations for someone who desperately wants a PCR machine but can't afford one and doesn't have the skills to delve into DIY electronics?

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/77f27357-12db-43a7-9dad-f89fa224b1ba%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/Xnnc0R_sy_E/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/b0a0961b-ab00-4b0d-8052-d3b7d8de5f61%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/Xnnc0R_sy_E/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/e8ea2580-92ad-4700-b6e5-33d9b5728b19%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/Xnnc0R_sy_E/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/e766d7c9-1476-42d0-8aaf-0e325e40d47e%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.