Re: [DIYbio] Re: least risky human hello world?

I agree, that would be a good approach if someone wanted to pursue this project in a professional setting.  I'm skeptical about the "easily" part, since the episomal vectors are usually larger and more difficult to transfect than a simple expression plasmid.  Not sure what the smallest/easiest episomal vector is though.

I'll add on one more requirement to my "hello world" list:
6)   Transfection should be carried out on a limited patch of tissue, such that even if every affected cell dies, there is little permanent damage/disability.

Responding to Cathal that there is no safe human hello world: 
I agree that real transhumanist, gene therapy stuff is much too difficult and dangerous for a DIY setting at this time.  However, a transient plasmid transfection on a limited patch of skin seems a lot less risky.  Not zero risk, but sometimes people take a risk doing something they are passionate about...I'd put it at lower risk than climbing everest or some of the home surgery the grinders are doing.

Of course I'm not doing any of those things myself :)