How about an ex vivo experiment? Not as dramatic but certainly safer. Pull out some of your own leukocytes or cheek epithelia and transfect them in a dish.
Yeah, cytokine storm and autoimmunity should be considered... My first-pass assessment is that chances are low for a small # of transfected cells and a well-studied protein. Luciferase has been used in many animal models, and although transfected cells may be eliminated by CD8+ response, I'm not aware of any reports of cross-reactivity or autoimmunity. I would definitely want to read up more and do more risk-assessment before trying it myself. I've been an immunology grad student for the last 3 years, but the immunology of gene therapy is pretty new to me.
-- Yeah, cytokine storm and autoimmunity should be considered... My first-pass assessment is that chances are low for a small # of transfected cells and a well-studied protein. Luciferase has been used in many animal models, and although transfected cells may be eliminated by CD8+ response, I'm not aware of any reports of cross-reactivity or autoimmunity. I would definitely want to read up more and do more risk-assessment before trying it myself. I've been an immunology grad student for the last 3 years, but the immunology of gene therapy is pretty new to me.
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