[DIYbio] Anyone else playing with electron/ion microscopes?

Been attending a workshop and they're just so cool! Wondering if there's anyone out there that does electron microscopy or FIB (focused ion beam) work for milling/sectioning or depositing material. There was a cool demo that used FreeCAD and a slicing script to generate milling or deposition patterns, basically what the 3D printer folks are doing. There was even a guy doing sectioning and imaging of cells which had been treated with nanoparticles, then frozen with cryo to allow sectioning. He was using the omniprobe to weld to the cells with water vapor, to pick them up and transfer them to a TEM grid (I think). The ice would eventually sublimate so the weld slop would go away to be able to get nice images. I could almost imagine the opposite, using this kind of thing to place frozen cells in some particle 3D/rotation arrangement.

Anyway, fun stuff!

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-Nathan

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