Hi everyone,
The DNA construct is synthesized and will be subcloned. Now I am desinging a version 2.0 (right, before v1 was tested. But I have the uppermost confidence it is going to work). My beekeeper in the US will do the actual transfection, so I am freed up to do some work on v2.
However, now I will need the "good old" CMV promoter. Does anyone know whether I can use it without it's enhancer? Will it still have the same expression pattern as the full lentgh promoter (probably not, that's why I am asking)?
CMV enhancer
GTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATG
CMV minimal promoter
GTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT
Usually it is nice to just take the full-lentgh promoter, knowing it will work as in the literature. But obviously, paying 200 bp more synthesis instead of 500 bp more (if it is unneccessary) makes a difference :D
I need to be sure about this, because I'd rather pay more if unsure if it would work.
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