I'm trying to learn more about the sanger sequencing machine we have. I have been trying to understand how the "sample submission" guidelines for plasmids/PCRs work. Is there a formula for how much template to use and how is it calculated?
For example, Genewiz says that for plasmids, you do: (10ng/ul)*(template length in kb). For PCR, its (2ng/ul)*(template length in kb). How did they come up with those numbers?-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
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