On Sat, Oct 11, 2014 at 3:38 AM, Josiah Zayner <josiah.zayner@gmail.com> wrote:
Yeah, I mean this should totally be possible. What chemicals are you missing? Maybe I can send them your way.
I think it was just the ammonium persulfate and the gelred that I needed.(gelred supposedly had better sensitivity, though their definition was loose and something like 'we can see a normal sized band in our imager with this mass ssDNA in a lane').
If you or others have the time to play with this stuff more, I'd be happy to send the sanger cycle sequencing kit and unpolymerized acrylamide to you (I could leave that out since you are already having some luck with agarose). This stuff has been in my freezer since Feb of last year, within a styrofoam cooler. I bet it's still good, but you'd want to check. I was just planning on using the included control primers and plasmid with short cycle times so extension wouldn't be too much, just so I could get better separation.
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