Re: [DIYbio] Anyone else playing with electron/ion microscopes?

On 10/09/2014 02:26 AM, Nathan McCorkle wrote:
> There was even a guy doing sectioning and imaging of cells which had been treated with nanoparticles, then frozen with cryo to
> allow sectioning.

How did sectioning work?

guess 1. Ablating layers and leaving the rest?
Was the focused beam able to get a smooth level surface, or depended
on time burning and then view and decide where to burn off more?

guess 2. Slicing through all and
using the flat surface left after rotating it 90 deg to view?

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