Drying can be done by washing the DNA with ethanol then warming with an open top in an oven.
--
-Nathan
-- If you're in a lab, and there are some cell/molbio folks around, you might try immortalizing some of your cells... but at that point why not just use an existing human cell line? There are only so many cells you can lose (from yourself) without noticing too much.
On Mon, Oct 13, 2014 at 11:44 PM, Johann <johtobsch@gmail.com> wrote:
Hello DIYbiologists,--
i planed to do (qualititativ) detection reactions of the atoms, which my DNA is build of. Therefor i need lots of DNA. To extract DNA i used the saltwater-dishcleaner-alcohol-method(used spiritus instead of iso-propanol, worked better in my case). The problem is that i also get lots of bacterial DNA using this method. Do you have any method to extract human DNA with much less bacteria DNA, without hurt and provide a good amount DNA per charge and can be done multiple times in a row. Due i have support from a chemistry teacher i can access some chemicals.
I do not ask for a method to replicate the DNA after extracted with nucleoacidsand thermocycler.
Do you have an idea for DNA extracting, which woks better in bigger scale than the described method?
Thanks for inspiration, literature, ideas or protocols
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-Nathan
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2 comments:
Well thank you. Sadly this problem is outdated know, but i did not used a heater to remove the water, but a freezer, it worked well too. The water just vanished, without the risk of harming the DNA by heat.
I did not picked a human cell line, since I had no access to a laboratory which was allowed to operate with this kind of cells and i were interested in human DNA, so i choose my own. Also i found out that DNA contains Carbon, Oxygen, Hydrogen, Nitrogen and Phosphorus by experiment. So it was a pointless experiment.
I also dropped DIYBio for other reasons.
It was quiet a funny time though. I somewhat miss it.
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