Re: [DIYbio] Looking for an Illumina Wizard

Cool, would have loved to see that!
It's a bit irrelevant to my needs, right now however. The basics of how
illumina works are widely known, it's the practicalities of how *well*
it works in nichey use-cases like mine that matter.

I've been told that it's unusual to multiplex more than 96 samples, and
that a few hundred (say 300) is really pushing the machine to its
limits. I'd like to hear if there's a credible dissenting view here,
because I thought Illumina could easily multiplex hundreds or thousand
of samples with reasonable coverage?

On 02/10/14 21:12, André Esteves wrote:
> http://ehsm.eu/index.html#IlluminaSequencer
>
> I was at this conference, but the presentation was not taped in fear of
> copyright and patent attacks...
> Sadly i don't remember the name of the presenter... Maybe you could contact
> the conference organizers and pass your request for contact...
>
> Cheers,
>
> Aife
>
> 2014-10-02 19:24 GMT+01:00 Cathal Garvey <cathalgarvey@cathalgarvey.me>:
>
>> Hey all,
>> I have an unusual project for which I'm planning to use Illumina as a
>> backend. However, the use-case is a novel repurposing of the platform,
>> and I'd really love to thresh it out with someone off-list.
>>
>> If you are, or know, someone with direct practical experience on an
>> Illumina MiSeq, I'd love to have a chat about factors like barcode
>> format and length, potential throughput, costings etcetera.
>>
>> Thanks!
>> Cathal
>>
>> --
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--
Twitter: @onetruecathal, @formabiolabs
Phone: +353876363185
Blog: http://indiebiotech.com
miniLock.io: JjmYYngs7akLZUjkvFkuYdsZ3PyPHSZRBKNm6qTYKZfAM