Re: [DIYbio] Re: 10% Agarose and 1-2 nucleobase resolution

Why be so condescending? Such as the only way it will be useful is if "this was done for R&D purposes though, then bravo." That's why I don't participate in this mailing list much any more. Too many condescending people. For what?

I thought I would share an idea and technique that people could work on and develop and perhaps be the basis for simple protocols to do DNA sequencing _AT HOME_. Is this not a _D_ _I_ _Y_ Bio mailing list?

Sure I can send off my stuff to be sequenced. Though $3 is on the low side unless you are doing a ton of samples or are at a university with in house sequencing(not available to DIY people). Most sequencing I pay for at NASA is ~$5 / sample roundtrip but maybe you know of a place including shipping that I can pay $3 a sample and have same-day return, please let me know. For a single sample, I would argue that this would be almost non-existent.

The cost of running a sequencing reaction and a 10% agarose gel as calculated by the materials I purchased is only ~$3.50-$4.50 ($0.20/gram of agarose at 5 grams($1) for 50mL and 10 grams($2) for 100mL). And that is not even the cheapest materials. I paid ~$100 for 200 units of polymerase and besides agarose that was probably the most expensive thing(~$2 / sequencing reaction). I opted for a good sequencing polymerase with no 5-3 exonuclease to make sure that it wasn't something holding back the experiments.

Again, money is not the point anyways. The point is to allow people to have access to these techniques at home or in a biohackerspace. People who do DIY Bio should already have the equipment needed to run these experiments. Obviously this needs to be developed more but I wanted people to be aware so if they wanted they could help develop it more.






On Fri, Oct 10, 2014 at 2:49 PM, 'SC' via DIYbio <diybio@googlegroups.com> wrote:
It's good to test the limits of processes that you use, but FYI, a single pass sequencing reaction (~$3) is probably cheaper than a 10% agarose gel.  If this was done for R&D purposes though, then bravo.

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