On Wed, Oct 15, 2014 at 11:49 AM, Johann <johtobsch@gmail.com> wrote:
Due common request my question in improved english:
Hello DIY-bios,
i am new to this mail list. To become familiar with the very basics of DIYBio, i try some very easy experiments. But since walking the same path, as somebody did before becomes quickly boring. I choose not only to extract DNA, which i did successful before, i am planning to do detection reactions for carbon, nitrogen, hydrogen, oxygen and phosphor. In the first step i want to show that these elements are part of my sample, in the second step i extend my experiment to do quantitative measurements. It is clear that these experiments will not change the world nor help anyone, but my self to get more familiar with the scientific method and experimenting. I am going to need at least for the second step a reasonable amount of DNA, to become precise results. The idea from McCorkel to ask some Cell-Bios does not help me. The reason is my environment, i am going to do this experiment in the chemistry lab of my school in some sort of chemistry-club. That is also why i can not archive SC's hilarious idea, which carry risk being kicked out from the group.
SC's idea was something I alluded to but didn't come out and say it... but really, what part of your humanity are you willing to give up to get this done? If cheek swab/ mouth rinse isn't sufficient, you only have the choice to cut a chunk of your flesh, bleed yourself, immortalize a cell line and grow it up, or use gametes which are dispensed in males quite easily. The issue of pH shouldn't matter, since you're interested in the DNA. Why not use a cell line? Or bacteria? Or yeast? Their DNA isn't know to be atomically different.
Does your school have an SEM with an EDX detector or a backscatter detector (and use ebsd)? Or a TEM and do electron diffraction spectroscopy. You can do chemical mapping with those techniques, with SEM you're probably limited to a few nanometers of resolution (and a double helix of DNA is only about 25nm wide), but won't likely get you single-atom data unless the microscope has a piezo stage which can move with sub-nanometer steps. The TEM would give you single-atom resolution, but in either case your data will be a bit muddled unless you straighten the DNA prior to viewing (with the water-air interface method). This paper gives that method and mentions a bit on TEM:
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