Hi everyone,
Just wondering if there is anyone with a lab (more or less) experienced with gibson assembly, who would like to participate in creating an open source vector. It is ought to be the coolest cloning vector ever.
I got my hands on a quite ancient piece of DNA (which is nowhere near outdated/obsolete).
Since the aec lab doesn't have competent coli in stock, nor ligase, nor gibson stuff, it would be great to outsource this "job".
Basically it should be quite easy, replacing a piece of pUC19 with my DNA. So 2 PCRs, and an assembly reaction. If we dare, we can even insert 2 pieces (making it 3 PCRs) to make the plasmid even better.
Just shoot me a mail if you have the free capabilities ;)
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