[DIYbio] Re: Isolating sequences from scratch

Hi Nico,
 
The cheapest way to get going would be to make a large insert shotgun library.  You'll need access to live plant tissue for that; if you don't have access to live plant tissue, you can make a small insert library.  That would be followed by some plasmid preps (not expensive), and some end-sequencing (a little expensive).  
 
I'd be happy to help you through the project. 
 
To start with, do you have access to live tissue?  Is this species wild-collected or cultivated?
 
Stacy

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