Re: [DIYbio] Re: Is there interest in lower cost restriction enzyme varieties?

This is why I was going to get into the "grow your own enzymes" market
instead with Glowbiotics. Making and selling purified, certifiable
enzymes is the sort of task that's economic-at-scale, which encourages a
natural monopoly to the largest and best established player.

There are plenty of groups selling ultra-cheap EcoRI, and some large
competition with NEB who go for quality over value, there's very little
room for a successful small player.

Instead, I thought there was room for a seller of grow-your-own kits,
starting with basic plasmids and working up to enzyme-production systems
that used trivial purification methods. Those who've seen me exposing
these at Synbio Future (http://www.indiebiotech.com/?p=245) will be
familiar with the Agar-based method I tried, but failed, to get to work
(let that count as prior art towards the idea of "using agar/ose/opectin
as a purification matrix". I still think this method could work but the
domain I was trying to use didn't play well with others.

It didn't turn out well for my plasmid plans, but it's possible that if
someone can work out a stack consisting of:
* Universally applicable, cheap, trivial purification (Is MBP out of
patent yet? Use with hot-filtered corn-starch to get MBP-bindable starch?).
* Universally applicable inducible expression standard, for production
of enzyme efficiently in culture.
* A library of well-characterised expression constructs including
preferred incubation conditions, perhaps leaning towards antha-lang.org
and OpenTrons for automated growth/induction/purification

..I think there'd be a modest market for that. But "modest" is key, I
made the mistake of overestimating this market in the past.

On 21/11/14 00:23, Dakota Hamill wrote:
> I've met with NEB about this same thing I go there once a month. their
> system has been perfected over 30 years. They do sell a lab starter
> pack with shrimp phosphatase, restriction enzymes, polymerase, ligase,
> etc. I'm on my phone and I can't find it. I'm not saying it's a bad
> idea because hell, I want to do the same thing. I came to realize I
> don't think the market is there. Sure maybe a few people on the list
> might help out or you could find some high schools, but no real lab is
> going to drop NEB for a cheaper enzyme seller who can't disclose they
> buy from NEB anyway.
>
> On Nov 20, 2014 4:47 PM, "'SC' via DIYbio" <diybio@googlegroups.com
> <mailto:diybio@googlegroups.com>> wrote:
>
> Justin,
>
> Maybe I misunderstood your intent. Were you planning on purifying
> your own enzymes or repackaging larger quantities from NEB into
> smaller aliquots?
>
> Stacy
>
>
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