Re: [DIYbio] Re: What analyses should I have performed on this tissue?

I suspect the difference is that organs for donation are still "viable";
live cells, active cytoplasm, ongoing RNA turnover and transcription,
however slow. After death without preservation, I think most relevant
tissues, particularly in the brain, would suffer cell death pretty
quickly, with RNA degradation being one of the fastest processes in that
chain of events.

Not being an expert on RNA metabolism or isolation though, I'm guessing
here.

On 14/12/14 00:03, Steve wrote:
> On Monday, 8 December 2014 18:01:33 UTC, SC wrote:
>
> ".....I'm not sure intact RNA can be retrived from a cadaver."
>
>
> on that point, any particular reason why not? at approximately 10
> picogram per mammalian cell, there is certainly enough of it. (And
> given that probing for viral RNA is used in screening donor organ
> tissue, which would be at much lower overall concentrations in cell
> lysates).
> Certainly at -80, useful data can be retrieved from tissue or lysates
> after years.
>
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